Copper transporter COPT5 participates in the crosstalk between vacuolar copper and iron pools mobilisation

Abstract : Copper (Cu) deficiency affects iron (Fe) homeostasis in several plant processes, including the increased Fe requirements due to cuproprotein substitutions for the corresponding Fe counterpart. Loss-of-function mutants from Arabidopsis thaliana high affinity copper transporter COPT5 and Fe transporters NATURAL RESISTANCE-ASSOCIATED MACROPHAGE PROTEIN 3/4 (NRAMP3 and NRAMP4) were used to study the interaction between metals internal pools. A physiological characterisation showed that the copt5 mutant is sensitive to Fe deficiency, and that nramp3nramp4 mutant growth was severely affected under limiting Cu. By a transcriptomic analysis, we observed that NRAMP4 expression was highly induced in the copt5 mutant under Cu deficiency, while COPT5 was overexpressed in the nramp3nramp4 mutant. As a result, an enhanced mobilisation of the vacuolar Cu or Fe pools, when the other metal export through the tonoplast is impaired in the mutants, has been postulated. However, metals coming from internal pools are not used to accomplish the increased requirements that derive from metalloprotein substitution under metal deficiencies. Instead, the metal concentrations present in aerial parts of the copt5 and nramp3nramp4 mutants conversely show compensated levels of these two metals. Together, our data uncover an interconnection between Cu and Fe vacuolar pools, whose aim is to fulfil interorgan metal translocation.
Document type :
Journal articles
Complete list of metadatas

Cited literature [56 references]  Display  Hide  Download

https://hal.archives-ouvertes.fr/hal-02104457
Contributor : Chantal Baracco <>
Submitted on : Friday, April 19, 2019 - 3:09:53 PM
Last modification on : Monday, November 18, 2019 - 3:46:53 PM

File

Carrió-Seguí, A.-et al Sci.R...
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Àngela Carrió-Seguí, Paco Romero, Catherine Curie, Stephane Mari, Lola Peñarrubia. Copper transporter COPT5 participates in the crosstalk between vacuolar copper and iron pools mobilisation. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.4648. ⟨10.1038/s41598-018-38005-4⟩. ⟨hal-02104457⟩

Share

Metrics

Record views

96

Files downloads

36