$^{64}$CuCl$_2$ PET Imaging of 4T1-Related Allograft of Triple-Negative Breast Cancer in Mice
Abstract
$^{64}$CuCl$_2$ is an economic radiotracer for oncologic PET investigations. In the present study, we characterized the uptake of $^{64}$CuCl$_2$ in vivo by µPET/CT in an allograft 4T1-related mouse model (BALB/c) of advanced breast cancer. 18F-FDG was used as a comparator. Twenty-two animals were imaged 7–9 days following 4T1-cell implantation inside mammary glands. Dynamic $^{64}$CuCl$_2$ µPET/CT acquisition or iterative static images up to 8 h p.i. were performed. Animal biodistribution and tumor uptake were first evaluated in vivo by µPET analysis and then assessed on tissue specimens. Concerning $^{18}$F-FDG µPET, a static acquisition was performed at 15 min and 60 min p.i. Tumor $^{64}$CuCl$_2$ accumulation increased from 5 min to 4 h p.i., reaching a maximum value of 5.0 ± 0.20 %ID/g. Liver, brain, and muscle $^{64}$CuCl$_2$ accumulation was stable over time. The tumor-to-muscle ratio remained stable from 1 to 8 h p.i., ranging from 3.0 to 3.7. Ex vivo data were consistent with in vivo estimations. The 18F-FDG tumor accumulation was 8.82 ± 1.03 %ID/g, and the tumor-to-muscle ratio was 4.54 ± 1.11. $^{64}$CuCl$_2$ PET/CT provides good characterization of the 4T1-related breast cancer model and allows for exploration of non-glycolytic cellular pathways potentially of interest for theragnostic strategies.
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