Actomyosin organelle functions of SPIRE actin nucleators precede animal evolution - UNICANCER
Journal Articles Communications Biology Year : 2024

Actomyosin organelle functions of SPIRE actin nucleators precede animal evolution

Martin Kollmar
Aishwarya Ravi
  • Function : Author
Noura Alzahofi
  • Function : Author
Klas Hatje
Asmahan Alghamdi
Jiyu Kim
  • Function : Author
Olena Pylypenko
Alistair N Hume
Eugen Kerkhoff
  • Function : Author
  • PersonId : 1067965

Abstract

An important question in cell biology is how cytoskeletal proteins evolved and drove the development of novel structures and functions. Here we address the origin of SPIRE actin nucleators. Mammalian SPIREs work with RAB GTPases, formin (FMN)-subgroup actin assembly proteins and class-5 myosin (MYO5) motors to transport organelles along actin filaments towards the cell membrane. However, the origin and extent of functional conservation of SPIRE among species is unknown. Our sequence searches show that SPIRE exist throughout holozoans (animals and their closest single-celled relatives), but not other eukaryotes. SPIRE from unicellular holozoans (choanoflagellate), interacts with RAB, FMN and MYO5 proteins, nucleates actin filaments and complements mammalian SPIRE function in organelle transport. Meanwhile SPIRE and MYO5 proteins colocalise to organelles in Salpingoeca rosetta choanoflagellates. Based on these observations we propose that SPIRE originated in unicellular ancestors of animals providing an actin-myosin driven exocytic transport mechanism that may have contributed to the evolution of complex multicellular animals.

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hal-04732698 , version 1 (11-10-2024)

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Martin Kollmar, Tobias Welz, Aishwarya Ravi, Thomas Kaufmann, Noura Alzahofi, et al.. Actomyosin organelle functions of SPIRE actin nucleators precede animal evolution. Communications Biology, 2024, 7, pp.832. ⟨10.1038/s42003-024-06458-1⟩. ⟨hal-04732698⟩
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