Skip to Main content Skip to Navigation
Journal articles

The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport

Abstract : This paper focuses on the nucleoprotein (NP) of the newly identified member of the Orthomyxoviridae family, Influenza D virus. To date several X-ray structures of NP of Influenza A (A/NP) and B (B/NP) viruses and of infectious salmon anemia (ISA/NP) virus have been solved. Here we purified, characterized and solved the X-ray structure of the tetrameric D/NP at 2.4 Å resolution. The crystal structure of its core is similar to NP of other Influenza viruses. However, unlike A/NP and B/NP which possess a flexible amino-terminal tail containing nuclear localization signals (NLS) for their nuclear import, D/NP possesses a carboxy-terminal tail (D/NPTAIL). We show that D/NPTAIL harbors a bipartite NLS and designed C-terminal truncated mutants to demonstrate the role of D/NPTAIL for nuclear transport.
Document type :
Journal articles
Complete list of metadatas

Cited literature [82 references]  Display  Hide  Download

https://hal.univ-grenoble-alpes.fr/hal-01997471
Contributor : Frank Thomas <>
Submitted on : Thursday, September 24, 2020 - 9:14:43 AM
Last modification on : Wednesday, October 14, 2020 - 4:15:35 AM

File

466968.pdf
Publisher files allowed on an open archive

Identifiers

Collections

Citation

Amélie Donchet, Justine Oliva, Alice Labaronne, Laura Tengo, Myriam Miloudi, et al.. The structure of the nucleoprotein of Influenza D shows that all Orthomyxoviridae nucleoproteins have a similar NPCORE, with or without a NPTAIL for nuclear transport. Scientific Reports, Nature Publishing Group, 2019, 9 (1), pp.600. ⟨10.1038/s41598-018-37306-y⟩. ⟨hal-01997471⟩

Share

Metrics

Record views

357

Files downloads

25