Lipopolythiourea Transfecting Agents: Lysine Thiourea Derivatives - Unité de pharmacologie chimique et génétique et d'imagerie Access content directly
Journal Articles Bioconjugate Chemistry Year : 2007

Lipopolythiourea Transfecting Agents: Lysine Thiourea Derivatives

Abstract

Synthetic vectors represent an alternative to recombinant viruses for gene transfer. We have recently explored the transfection potential of a class of noncationic lipids bearing thiourea moieties as DNA associating headgroups. The encouraging results obtained with lipopolythioureas derived from serinol prompted us to investigate further this family of vectors. In the present study, we considered the transfection properties of a series of derivatives based on a different thiourea polar headgroup bearing a lysine scaffold. The synthesis of these compounds could be readily achieved in 3 steps with good yields. We found that these lipopolythioureas (LPT) might be considered as alternative systems for gene transfection, since their activity reached the same magnitude range as cationic vectors in the presence of serum. LPT with 14-carbon length chains appeared to be more efficient as transfecting agent than the ones with shorter chains. Toxicity studies proved that the hydration film 2 method led to particles well tolerated both by the cells in vitro and by the mice in vivo. The ability to induce gene expression in vivo was demonstrated by intratumoral injection. Finally, biodistribution studies showed that the quantity recovered in the blood circulation, 2h after systemic injection, was improved as compared to cationic lipids.
Fichier principal
Vignette du fichier
bc-2007-001924_revised manuscript_140907.pdf (1.01 Mo) Télécharger le fichier
Origin : Files produced by the author(s)
Loading...

Dates and versions

hal-02512550 , version 1 (23-03-2020)

Identifiers

Cite

Jeanne Leblond, Nathalie Mignet, Celine Largeau, Johanne Seguin, Daniel Scherman, et al.. Lipopolythiourea Transfecting Agents: Lysine Thiourea Derivatives. Bioconjugate Chemistry, 2007, ⟨10.1021/bc7001924⟩. ⟨hal-02512550⟩
46 View
98 Download

Altmetric

Share

Gmail Facebook X LinkedIn More