Gut-specific actinobacterial community structure and diversity associated with the wood-feeding termite species, Nasutitermes corniger (Motschulsky) described by nested PCR-DGGE analysis

Thomas Lefebvre 1 Edouard Miambi 1 Anne Pando 1 Michel Diouf 1 Corinne Rouland-Lefèvre 1, *
* Corresponding author
1 Bioemco - Biogéochimie et écologie des milieux continentaux
Abstract : This comprehensive survey studied the actinobacterial community structure and putative representative members associated with the gut of the wood-feeding termite, Nasutitermes corniger (Motschulsky), using nested PCR-DGGE and 16S rDNA sequences analyses. The closest relatives of the actinobacteria inhabiting the gut of Nasutitermes corniger were in five families, regardless of the geographical origin of the termite colony: Propionibacteriaceae, Streptomycetaceae, Cellulomonodaceae, Corynebacteriaceae and Rubrobacteraceae. Feeding termites on beech wood did not result in substantial changes in the actinobacterial community structure as revealed by DGGE banding patterns. Most of the 16S rDNA sequences obtained after excision and sequencing of DGGE bands clustered with those previously retrieved in termite guts. These results confirm the presence of gut-specific actinobacteria. Except for the 16S rDNA sequences affiliated to Streptomycetaceae and Cellulomonodaceae, no sequence had more than 97% similarity with the closest isolated strains, indicating the presence of microorganisms that have not yet been cultivated. These results suggest that members of the Actinomycetales order account for the largest proportion of the Actinobacteria phylum inhabiting the gut of the termite N. corniger.
Keywords : Actinomycetes Termite gut. Nested PCR DGGE
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Journal articles
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https://hal-bioemco.ccsd.cnrs.fr/bioemco-00444897
Contributor : Anne Pando <>
Submitted on : Thursday, January 7, 2010 - 2:42:51 PM
Last modification on : Sunday, October 20, 2019 - 1:24:32 AM

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Thomas Lefebvre, Edouard Miambi, Anne Pando, Michel Diouf, Corinne Rouland-Lefèvre. Gut-specific actinobacterial community structure and diversity associated with the wood-feeding termite species, Nasutitermes corniger (Motschulsky) described by nested PCR-DGGE analysis. Insectes Sociaux, Springer Verlag, 2009, 56, pp.269-276. ⟨10.1007/s00040-009-0020-6⟩. ⟨bioemco-00444897⟩

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