UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells - Immunologie-Immunopathologie-Immunothérapie Access content directly
Journal Articles Nature Communications Year : 2017

UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells

Sophia Maschalidi
  • Function : Author
Paula Nunes-Hasler
Clarissa R Nascimento
  • Function : Author
Ignacio Sallent
  • Function : Author
Valérie Lannoy
  • Function : Author
Meriem Garfa-Traore
  • Function : Author
Nicolas Cagnard
  • Function : Author
Fernando E Sepulveda
Pablo Vargas
Ana-Maria Lennon-Duménil
  • Function : Author
Peter van Endert
Thierry Capiod
  • Function : Author
Nicolas Demaurex
Bénédicte Manoury
  • Function : Author
  • PersonId : 919752

Abstract

Abstract Dendritic cells (DC) have the unique ability to present exogenous antigens via the major histocompatibility complex class I pathway to stimulate naive CD8 + T cells. In DCs with a non-functional mutation in Unc93b1 (3d mutation), endosomal acidification, phagosomal maturation, antigen degradation, antigen export to the cytosol and the function of the store-operated-Ca 2+ -entry regulator STIM1 are impaired. These defects result in compromised antigen cross-presentation and anti-tumor responses in 3d-mutated mice. Here, we show that UNC93B1 interacts with the calcium sensor STIM1 in the endoplasmic reticulum, a critical step for STIM1 oligomerization and activation. Expression of a constitutively active STIM1 mutant, which no longer binds UNC93B1, restores antigen degradation and cross-presentation in 3d-mutated DCs. Furthermore, ablation of STIM1 in mouse and human cells leads to a decrease in cross-presentation. Our data indicate that the UNC93B1 and STIM1 cooperation is important for calcium flux and antigen cross-presentation in DCs.
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Dates and versions

hal-04059179 , version 1 (05-04-2023)

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Sophia Maschalidi, Paula Nunes-Hasler, Clarissa R Nascimento, Ignacio Sallent, Valérie Lannoy, et al.. UNC93B1 interacts with the calcium sensor STIM1 for efficient antigen cross-presentation in dendritic cells. Nature Communications, 2017, 8 (1), pp.1640. ⟨10.1038/s41467-017-01601-5⟩. ⟨hal-04059179⟩
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