Synthesis of Double Hydrophilic Block Copolymers Poly(2‐isopropyl‐2‐oxazoline‐ b ‐ethylenimine) and their DNA Transfection Efficiency - Plateforme RMN - Sorbonne Université Access content directly
Journal Articles Macromolecular Bioscience Year : 2022

Synthesis of Double Hydrophilic Block Copolymers Poly(2‐isopropyl‐2‐oxazoline‐ b ‐ethylenimine) and their DNA Transfection Efficiency

Abstract

Gene delivery is now a part of the therapeutic arsenal for vaccination and treatments of inherited or acquired diseases. Polymers represent an opportunity to develop new synthetic vectors for gene transfer, with a prerequisite of improved delivery and reduced toxicity compared to existing polymers. Here, the synthesis in a two‐step's procedure of linear poly(ethylenimine‐ b‐ 2‐isopropyl‐2‐oxazoline) block copolymers with the linear polyethylenimine ( l PEI) block of various molar masses is reported; the molar mass of the poly(2‐isopropyl‐2‐oxazoline) (PiPrOx) block has been set to 7 kg mol −1 . Plasmid DNA condensation is successfully achieved, and in vitro transfection efficiency of the copolymers is at least comparable to that obtained with the l PEI of same molar mass. l PEI‐ b ‐PiPrOx block copolymers are however less cytotoxic than their linear counterparts. PiPrOx can be a good alternative to PEG which is often used in drug delivery systems. The grafting of histidine moieties on the l PEI block of l PEI‐ b ‐PiPrOx does not provide any real improvement of the transfection efficiency. A weak DNA condensation is observed, due to increased steric hindrance along the l PEI backbone. The low cytotoxicity of l PEI‐ b ‐PiPrOx makes this family a good candidate for future gene delivery developments.
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Dates and versions

hal-04265776 , version 1 (03-11-2023)

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Gwendoline Delecourt, Laetitia Plet, Yann Le Guen, Ozgul Tezgel, Guillaume Tresset, et al.. Synthesis of Double Hydrophilic Block Copolymers Poly(2‐isopropyl‐2‐oxazoline‐ b ‐ethylenimine) and their DNA Transfection Efficiency. Macromolecular Bioscience, 2022, 23 (1), pp.2200296. ⟨10.1002/mabi.202200296⟩. ⟨hal-04265776⟩
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