Multivariate analysis of protein profiles of metal hyperaccumulator Thlaspi caerulescens accessions - Bioemco
Article Dans Une Revue Proteomics Année : 2006

Multivariate analysis of protein profiles of metal hyperaccumulator Thlaspi caerulescens accessions

Marjo H. Tuomainen
  • Fonction : Auteur
Satu J. Lehesranta
  • Fonction : Auteur
Arja I. Tervahauta
  • Fonction : Auteur
Viivi H. Hassinen
  • Fonction : Auteur
Henk Schat
  • Fonction : Auteur
Kaisa M. Koistinen
  • Fonction : Auteur
Seppo Auriola
  • Fonction : Auteur
Jim W. Mcnicol
  • Fonction : Auteur
Sirpa O. Kärenlampi
  • Fonction : Auteur

Résumé

Thlaspi caerulescens is increasingly acknowledged as one of the best models for studying metal hyperaccumulation in plants. In order to study the mechanisms underlying metal hyperaccumulation, we used proteomic profiling to identify differences in protein intensities among three T. caerulescens accessions with pronounced differences in tolerance, uptake and root to shoot translocation of Zn and Cd. Proteins were separated using two-dimensional electrophoresis and stained with SYPRO Orange. Intensity values and quality scores were obtained for each spot by using PDQuest software. Principal component analysis was used to test the separation of the protein profiles of the three plant accessions at various metal exposures, and to detect groups of proteins responsible for the differences. Spot sets representing individual proteins were analysed with the analysis of variance and non-parametric Kruskal-Wallis test. Clearest differences were seen among the Thlaspi accessions, while the effects of metal exposures were less pronounced. The 48 tentatively identified spots represent core metabolic functions (e.g. photosynthesis, nitrogen assimilation, carbohydrate metabolism) as well as putative signalling and regulatory functions. The possible roles of some of the proteins in heavy metal accumulation and tolerance are discussed

Dates et versions

bioemco-00136960 , version 1 (16-03-2007)

Identifiants

Citer

Marjo H. Tuomainen, Naoise Nunan, Satu J. Lehesranta, Arja I. Tervahauta, Viivi H. Hassinen, et al.. Multivariate analysis of protein profiles of metal hyperaccumulator Thlaspi caerulescens accessions. Proteomics, 2006, 6, pp.3696-3706. ⟨10.1002/pmic.200501357⟩. ⟨bioemco-00136960⟩
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